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KMID : 0366119770050010166
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Korean Journal of Applied Microbiology & Bioengineering
1977 Volume.5 No. 1 p.166 ~ p.177
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Taxonomic Studey of Bacillus coagulans by Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization Technique
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Chung Chi-Kwan
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Abstract
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Taxonomic study of 11 strains of Bacillus coagulans and 14 strains of 13 species of Bacillus by deoxyribonucleic acid (DNA)-DNA hybridization were conducted. Among the 11 strains of B. coagulans, 6 were isolated from soil and the rest were the authentic strains obtained from American Type culture collection (ATCC) or the Institute for Fermentation, Osaka (IFO). All strains were examined to confirm as they are expected species of B. coagulans by the methods of Gordon et al. according to Bergey´s Manual.
(8th ed.).
The intraspecific DNA homology indexes among the 11 strains of B. coagulans using strain ATCC 7050 as the. standard (^(3)H labeled input DNA) showed 76 % or, more, respectively. These findings accorded well with the results of the conventional taxonomic study according to the Bergey´s Manual.
The interspecific DNA homology indexes between B. coagulans strain ATCC 7050 and the type cultures of B. subtilis (168), B. lichenformis (IFO 12107), B. pumilus (IFO 12110), B. firmus (ATCC 145 75), B. lentus (ATCC 10840), B. circulans (ATCC 4513), B. macelans (ATCC 8244), B. polymyxa (ATCC 842), B. sphaericus (ATCC 14577), B. brevis (ATCC 8246, IFO 12334), B. laterosporus (ATCC 64), and B. pantothenticus (ATCC 14576) respectively, showed 2 to 4%, while that of between B. coagulans ATCC 7050 and Escherichia coli K-12 was less than 1 %.
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